Fluorescence Adapter for Hirox Digital Microscope
The NIGHTSEA Model SFA Fluorescence Adapter system can add a versatile fluorescence imaging capability to the Hirox digital microscope. To see examples of images made with Hirox systems visit our Hirox image gallery.
Fluorescence solutions for Hirox lenses
Our adaptations to the Hirox system are concerned with the lens. We now offer variants of our fluorescence adapter system that work with the HR-2500E and HR-1020E lenses. (We may be able to support older lenses – contact NIGHTSEA for more information.)
The key elements of any fluorescence system are:
- a light source that produces sufficient energy in the appropriate wavelength range to excite fluorescence in the sample of interest;
- a barrier filter in the viewing path that blocks reflected excitation light while transmitting the fluorescence emitted by the sample.
NIGHTSEA implements these for the Hirox system with:
- high intensity LED light sources available in five excitation wavelength ranges (see list further down this page);
- emission barrier filters that can be easily added to the Hirox lenses
- The filters for the HR-2500E and HR-1020E lenses simply insert into the Analyzer slot
The components of a NIGHTSEA system that adds a single excitation/emission wavelength combination on a Hirox microscope are:
- Flexible gooseneck lamp base with LED drive circuit and intensity control;
- Universal power supply (120/240VAC, 50/60Hz) with international plug set
- Excitation light source
- Emission barrier filter
- Barrier filter glasses for fluorescence viewing and eye safety
- Padded carrying case
Additional wavelength sets are implemented by adding a matched set (light plus filter set) consisting of a light source, barrier filter, and filter glasses.
Order the adapter systems for Hirox on the Electron Microscopy Sciences (EMS) web site.
(Click image for larger view)
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- Hirox HR-1020E lens with NIGHTSEA UV illuminator
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- Royal Blue barrier filter for HR-2500E lens
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- Barrier filter installed in HR-2500E lens
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- Concrete sample, HR-2500E 140x ring light
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- Concrete sample, HR-2500E 140x fluorescence with UV
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- Travel document, HR-2500E 30x ring light
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- Travel document, HR-2500E 30x fluorescence with UV
We are currently working on adapters for the full range of Hirox lenses, for use with all of our wavelength options. Please contact Hirox or NIGHTSEA to discuss your application and compatible solutions.
Wavelength sets
There are five excitation/emission wavelength combinations available for the system.
| Color | Designation | Excitation | Emission |
| Ultraviolet | UV | 360 – 380nm | 415nm longpass |
| Violet | VI | 400 – 415nm | 450nm longpass |
| Royal Blue | RB | 440 – 460nm | 500nm longpass |
| Cyan | CY | 490 – 515nm | 550nm longpass |
| Green | GR | 510 – 540nm | 600nm longpass |
Order the adapter systems for Hirox on the Electron Microscopy Sciences (EMS) web site.