Application Note #3 – NIGHTSEA helps U Montana’s exciting spectrUM Discovery Area uncover the hidden world of neuroscience
Growing with neuroscience
As children mature, their eyes are able to adjust to looking through the binoculars on a microscope and fluorescence experiments grow to keep pace. Using NIGHTSEA’s newly-developed Stereo Microscope Fluorescence Adapter (SFA), any stereo microscope now can be fitted for fluorescence.

Using the Stereo Microscope Fluorescence Adapter (SFA) moves the study of fruit fly larvae to the next level. The three panels on the right illustrate selective use of GFP to highlight (a) the actin filaments which are pervasive through the larvae, (b) the peripheral and central nervous system, and (c) the salivary glands and cells that will develop into the eyes.
As shown in the figure, the SFA consists of an easy-to-use LED light source mounted on a goose neck, a barrier filter magnetically mounted beneath the objectives, and a light shield. Blue excitation was chosen for this experiment to best illuminate GFP, however the SFA also comes equipped for violet, cyan, and green excitation, as well as a white light illuminator.
See how spectrUM uses NIGHTSEA technology for neuroscience for young kids, older kids, and on the road